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The peptide was first identified in 1973 by Loren Pickart during research on human plasma, where he observed that a small copper-peptide complex was present in younger blood samples at higher concentrations than in older ones

The biochemistry behind this process involves testosterone conversion through a specific enzyme

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Arch Biochem Biophys 302:348355 Shaik IH, Mehvar R (2006) Rapid determination of reduced and oxidized glutathione levels using a new thiol-masking reagent and the enzymatic recycling method: application to the rat liver and bile samples